Laboratory Technician (Biochemistry & Molecular Biology)

Join the University of Sheffield for an outstanding opportunity in groundbreaking drug development research. You can contribute to the fight against antibiotic-resistant bacteria by providing technical support to a small team of scientists working an exciting discovery program. We seek an enthusiastic co-worker to carry out a range of techniques targeting a pathogen called Pseudomonas aeruginosa.

As the team's laboratory technician, you will primarily be responsible for providing high-quality protein reagents for your colleagues as well as implementing sophisticated assays developed within the group. You will also carry out cell-based assessments on bacterial and mammalian cells to determine their responses to our molecules. Typical responsibilities will be:

• Perform standard inhibitor assays (biochemical and cell-based).
• Purify bacterial FEN protein using FPLC chromatography.
• Express and purify non-radioactive isotopically labelled derivatives.
• Co-ordinate research activities with our NMR structural biologist.
• Contribute to preparation of research reports, publications, and presentations.
• Participate in laboratory meetings, seminars, and conferences to present your findings.
• Construct recombinant plasmids, carry out routine immunoassays.
• Analyse protein preparations by SDS-PAGE.
• Setting up crystallization trial with inhibitors.

To be successful in this role, you must have relevant academic qualifications and laboratory experience in the following techniques; cloning, PCR, recombinant protein production, use of FPLC chromatography system, cell culture skills (bacterial and mammalian), experience of working in an ISO9001 laboratory, immediate availability . You will also be familiar with basic chemo-informatics and drug screening tools.

You will join a team of researchers led by Prof. Jon Sayers, which includes structural biologists dedicated to identifying new drugs to treat disease. The team has access to brand new protein purification and analytical services, modern labs, and the range of equipment and facilities required to conduct innovative research. This is an outstanding chance to work in a dynamic and encouraging environment, where your contributions can make a significant impact on global health.

If you are passionate about scientific discovery and eager to contribute to world-class research, we encourage you to apply. Please submit your CV, a cover letter outlining your relevant experience and why you are interested in this position and contact information for three professional references. Applications will be reviewed on a rolling basis, so early submission is highly encouraged.

Further information can be found at https://www.sheffield.ac.uk/jobs/benefits.

We are seeking suitably experienced candidates with relevant academic qualifications who are able to start immediately. As this role involves supporting both structural biology and biochemistry/cell biology aspects of the project, the ideal candidate will also have experience in chemo-informatics software such as DataWarrior and PyRx (Autodock).

We build teams of people from different heritages and lifestyles from across the world, whose talent and contributions complement each other to greatest effect. We believe diversity in all its forms delivers greater impact through research, teaching and student experience.

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